mesoplasma florum l1 atcc  (ATCC)

Journal: Applied and Environmental Microbiology

Article Title: Development of oriC -Based Plasmids for Mesoplasma florum

doi: 10.1128/AEM.03374-16

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: However, in contrast to the M. florum oriC -based pMflT-o4 plasmid, none of the new heterologous oriC constructs yielded any tetracycline-resistant colony when transformed in M. florum . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain or plasmid Relevant genotype or phenotype a Source or reference Strains Escherichia coli EC100D pir + F − mcrA Δ (mrr-hsdRMS-mcrBC) φ 80dlacZ ΔM15 Δ lacX74 recA1 endA1 araD139 Δ (ara leu)7697 galU galK λ − rpsL nupG pir + ( DHFR ) (Sm r ) Epicentre MFD pir MG1655 RP4-2-Tc::[ΔMu1:: aac(3)IV -Δ aphA -Δ nic35 -ΔMu2:: zeo ] Δ dapA ::( erm-pir ) Δ recA (Apra r Zeo r Em r ) 35 MM294 F − glnX44(AS) λ − endA1 spoT1 thiE1 hsdR17 creC510 E. coli Genetic Stock Center (strain 6315) Mesoplasma florum L1 ATCC 33453 L1 clone 3632 mfl169 ::Tn- tetM This study Plasmids pMflT-o1 ColE1 oriT RP4 M. florum oriC1 tetM (Tc r ) This study pMflT-o2 ColE1 M. florum oriC2 tetM (Tc r ) This study pMflT-o3 ColE1 oriT RP4 M. florum oriC3 tetM (Tc r ) This study pMflT-o4 ColE1 oriT RP4 M. florum oriC4 tetM (Tc r ) This study pMflCT-o4 ColE1 oriT RP4 M. florum oriC4 tetM cat (Tc r Cm r ) This study pMflET-o4 ColE1 oriT RP4 M. florum oriC4 tetM ereB (Tc r Em r ) This study pMflPT-o4 ColE1 oriT RP4 M. florum oriC4 tetM pac (Tc r Pu r ) This study pMflST-o4 ColE1 oriT RP4 M. florum oriC4 tetM aadA1 (Tc r Sp r Sm r ) This study pMYCO1 ColE1 M. mycoides subsp. capri oriC tetM bla (Tc r Ap r ) 24 pMYSO1 ColE1 M. mycoides subsp. mycoides oriC tetM bla (Tc r Ap r ) 24 pMCO3 ColE1 M. capricolum subsp. capricolum oriC tetM bla (Tc r Ap r ) 24 pSD4 ColE1 S. citri oriC tetM bla (Tc r Ap r ) 25 pMmcT ColE1 oriT RP4 M. mycoides subsp. capri oriC tetM (Tc r ) This study pMmmT ColE1 oriT RP4 M. mycoides subsp. mycoides oriC tetM (Tc r ) This study pMcapT ColE1 oriT RP4 M. capricolum subsp. capricolum oriC tetM (Tc r ) This study pSciT-o4 ColE1 oriT RP4 S. citri oriC tetM (Tc r ) This study ereB -pUC57 ColE1 bla ereB (Ap r Em r ) This study pTT01 ColE1 tetM (Tc r ) This study pUC19 ColE1 bla (Ap r ) 66 pSW23T R6K oriT RP4 cat (Cm r ) 67 Open in a separate window a DHFR , dihydrofolate reductase gene; Sm r , streptomycin resistant; Apra r , apramycin resistant; Zeo r , zeocin resistant; Em r , erythromycin resistant; Tc r , tetracycline resistant; Cm r , chloramphenicol resistant; Pu r , puromycin resistant; Sp r , spectinomycin resistant; Ap r , ampicillin resistant.

Techniques: Plasmid Preparation